Prophase in mitosis is the first stage of the cell cycle during which chromosomes condense into chromatin. There are three main stages: interphase, prophase, and metaphase. During metaphase, two molecules called kinetochores attach to centromeres on opposite ends of the chromosome, forming a spindle that separates the sister chromatids. For example, with two sets of DNA strands emerging from one set of nuclei; each strand is composed of two chains joined at their 5' ends but separated at their 3' ends by a protein bridge (the spindle). The centrosomes then attach to either side of the spindle and initiate microtubule assembly.
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